. 2023 Jun 9;27(7):1384–1389. doi: 10.1021/acs.oprd.3c00135

Table 1. Influence of H₂O₂ Feeding Rate and Enzyme Concentration on a 100 mL Scale^a.

experiment	[AaeUPO] [μM]	[cyclohexane] [mM]	H₂O₂-feeding rate [mM h^–1]	time [h]	total product concentration [mM]	productivity [g L^–1 h^–1]	turnover number (TN = mol_Product × mol_rAaeUPO^–1)
1	10	500	50	24	70.7	0.3	7000
2	20	500	50	24	390	1.6	19,500
3	20	500	200	4	373	9.3	18,650
4	20	3 × 500 (0, 6, 28 h)	20	24	269	1.1	13,450

Reaction conditions: [substrate] = 500 mM, [KPi, pH 6] = 100 mM, [ACN] = 50 vol %, [AaeUPO] = 10–20 μM, [H₂O₂-feeding rate] = 20–200 mM h^–1 (4–17.6 M aqueous stock solution) starting volume 100 mL, 25 °C, 300 rpm, duplicate measurements. The detailed time courses are listed in the Supporting Information (Figures S1–S4).

Table 1. Influence of H2O2 Feeding Rate and Enzyme Concentration on a 100 mL Scalea.

Table 1. Influence of H₂O₂ Feeding Rate and Enzyme Concentration on a 100 mL Scale^a.