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Fluorescence emission titration spectra of BSA, measured at room temperature: (a) 1 with [BSA] = 1.39 μM and [1]:[BSA] = 0.0–2.1; (b) 2 with [BSA] = 1.15 μM and [2]:[BSA] = 0.0–3.4; (c) 3 with [BSA] = 1.35 μM, and [3]:[BSA] = 0.0–3.4; and (d) 4 with [BSA] = 1.33 μM and [4]:[BSA] = 0.0–2.9. In all systems, progressive quenching (emission intensity decrease) was observed with the addition of the solutions containing 1–4.
