Figure 1.
FGF3 expressed in SBC and primary cell culture. (A) The agarose gel electrophoresis results of three groups of DNA samples extracted from SBC and DNA markers. (B) Western blot analysis of three sets of normal breast tissue and SBC protein samples (a). The expression of FGF3 in SBC and normal breast tissues was quantified and demonstrated by a histogram (b); each bar represents the mean ± standard deviation of three replicates (**P <0.01). (C) Morphological characteristics of primary cell mixed cell colony (a) and MA-891 cells (b). (D) H&E staining of MA-891 (a) and TA-1106 cells (b). (E) Western blot results showed expression levels of FGF3 in MA-891, TA-1106 and cells from a second clone (a). A histogram showing the quantitative results of FGF3 expression in MA-891, TA-1106 cells and cells from a second clone. FGF3, fibroblast growth factor 3; SBC, spontaneous breast cancer; MMTV, mouse mammary tumor virus; TA2, Tientsin albino 2.