Figure 6.

Overlapping nature of the four transcript amplification products. Transcript-4 is the smallest amplification product nested within the other three to be used as a probe to equally detect all four transcripts. Downstream primers are anchored in the poly (A) tail of the message by a series of ten T residues. Thus only the first 8 nucleotides of each downstream primer correspond to nucleotides in the 3' end of the subunit A gene. Primer pair T-11 and T-12 amplifies a 579 base pair fragment corresponding to transcript-1. Primer pair T-21 and T-22 amplifies a 455 base pair fragment corresponding to transcript-2. Primer pair T-31 and T-32 amplifies a 362 base pair fragment corresponding to transcript-3. Primer pair T-41 and T-42 amplifies a 238 base pair fragment corresponding to transcript-4.