Fig. 1 |. Activity of Bl_Man38A, Bl_Man38B and Bl_Man38C on native substrates and disaccharides.

a,b, Assays of Bl_Man38A–Bl_Man38C supplemented with 0.1 mM CoCl₂ on Man₅GlcNAc (a) or Man₉GlcNAc (b) in 60-min reactions. The asterisk (*) indicates internal control (xylohexaose). c–f, Kinetic assays of Bl_Man38A (c), Bl_Man38B (d), Bl_Man38C (e) and Bl_Man125 (f) on the disaccharides α-1,2-mannobiose (dark red), α-1,3-mannobiose (pink) and α-1,6-mannobiose (blue). The reactions containing GH38 α-mannosidases were supplemented with 0.1 mM CoCl₂. Results are expressed as mean ± s.d. from three independent experiments. For Bl_Man125, only the kinetics curve for α-1,6-mannobiose is shown because no activity was detected with the other disaccharides. Mass spectra are provided in Supplementary Figs. 2–4. Kinetic parameters for the disaccharide hydrolysis are shown in Supplementary Table 4.