Extended Data Fig. 5 |. The complementary activity of Bl_Man125 with Bl_Man38A-C enzymes on Man₅GlcNAc.

Reactions combining the GH125 α-1,6-mannosidase and the GH38 enzymes using Man₅GlcNAc to evaluate a putative booster effect in the degradation of small oligosaccharides. a, Negative control (no enzyme). b, Bl_Man125, c, Bl_Man38A, d, Bl_Man38A and Bl_Man125, e, Bl_Man38B, f, Bl_Man38B and Bl_Man125, g, Bl_Man38C, h, Bl_Man38C and Bl_Man125, i, Bl_Man38A and Bl_Man38C, j, Bl_Man38A, Bl_Man38C and Bl_Man125. Note that a remarkable difference is observed for Bl_Man38B, in which peaks corresponding to Man₃GlcNAc and Man₂GlcNAc are depleted in the presence of Bl_Man125.