Extended Data Fig. 7 |. Maximum likelihood phylogeny of characterized members of the GH38 family.

Red circles indicate the sequences containing at least one structure deposited in the Protein Data Bank, with their respective codes assigned. The yellow clade indicates bacterial GH38 α-mannosidases that oligomerizes as globular dimers and is represented by the structure under the PDB code 2WYH. The purple clades correspond to lysosomal and vacuolar (for plants) family members, which oligomerize as elongated dimers as shown for the structure under the PDB code 1O7D. The green clade corresponds to monomeric enzymes that are anchored to Golgi apparatus membrane, represented by the structure under the PDB code 1HTY. The red clades comprise the tetrameric enzymes, grouping cytosolic, vacuolar (for yeasts) and bifidobacterial members in the same phylogenetic branch. Grey clades do not have structural data available to infer the oligomeric state of their members. A GH92 α-mannosidase was used as the outgroup.