Extended Data Fig. 8 |. Functional comparison between the mutant Bl_Man38C_F726W and the wild-type Bl_Man38C.

Assays using Man₅GlcNAc (a–c) and Man₉GlcNAc (d–f) as substrate, in 60-min reactions with no enzyme (a, d), with Bl_Man38C (b, e) and with Bl_Man38C_F726W (c, f). *: internal control (xylohexaose). g–i, Kinetic assays with Bl_Man38C and Bl_Man38C_F726W on the disaccharides α-1,2-mannobiose (g), α-1,3-mannobiose (h) and α-1,6-mannobiose (i). The reactions were supplemented with 0.1 mM CoCl₂. Results are expressed as mean ± SD from three independent experiments (g–i).