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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Nat Chem Biol. 2022 Nov 28;19(2):218–229. doi: 10.1038/s41589-022-01202-4

Fig. 4 |. Cleavage profile of high-mannose N-glycans by Bl_Endo85 and Bl_Glc31.

Fig. 4 |

a, Control of GlcMan9GlcNAc2 in which no enzymes were added. b, Reaction of Bl_Endo85 against GlcMan9GlcNAc2, generating GlcMan9GlcNAc. c, Reaction of Bl_Glc31 against GlcMan9GlcNAc, generating Man9GlcNAc. d,f, Controls of Man5GlcNAc2-Asn (d) and Man9GlcNAc2-Asn (f) in which no enzymes were added. e,g, Reactions of Bl_Endo85 against Man5GlcNAc2-Asn (e) and Man9GlcNAc2-Asn (g), generating Man5GlcNAc and Man9GlcNAc, respectively. Asterisks (*) indicate the internal control xylohexaose. m/z values are indicated above the substrates or reaction products, and all the mass spectra are provided in Supplementary Figs. 20 and 21.