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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Nat Chem Biol. 2022 Nov 28;19(2):218–229. doi: 10.1038/s41589-022-01202-4

Fig. 5 |. bl1337 encodes an efficient ManI.

Fig. 5 |

a, Gene cluster including bl1337 (ManI Bl_MI), bl1339 (fructokinase), bl1338 (GH125 α-1,6-mannosidase) and bl1340 (ROK-family transcriptional regulator); a, biochemically characterized in this work; b, characterized in a previous work28; c, activity predicted. b, Substrate saturation curve of Bl_MI including kinetic parameters. Results are expressed as mean ± s.d. from three independent experiments (n = 3). c, Proposed biochemical route for the conversion of mannose to fructose-6-phosphate, a central molecule for the fermentative pathway. d, Phylogenetic tree of Bifidobacterium genomes, indicating the presence of genes encoding ManIs, mannose-6-phosphate isomerases, GH5_18 β-mannosidases (markers for N-glycan degradation) and GH26 and GH5_8 β-mannanases (markers for mannan/glucomannan degradation). Multiple species are detailed in Extended Data Fig. 2.