Skip to main content
. Author manuscript; available in PMC: 2023 Dec 12.
Published in final edited form as: Cancer Cell. 2022 Dec 12;40(12):1470–1487.e7. doi: 10.1016/j.ccell.2022.11.006

Figure 2: T-Cell Receptor Knockout in Uni-Vect CAR-T cells.

Figure 2:

(A) Uni-Vect-CAR-T cells may be stimulated by either the introduced CAR or the endogenous TCR. Endogenous TCR knockout prevents expression of the accessory molecule induced by endogenous TCR signaling. (B) TCR knockout in pASP85-CAR-T cells. NFAT signaling induced by 24 h stimulation with PMA+ionomycin, anti-CD3/CD28 dynabeads, or target SKOV3 cells was monitored in TCR+ and TCR− CAR-T cells by flow cytometry via built-in NFAT-eGFP inducible module. (C) MFI quantification of NFAT-inducible eGFP in Q1 and Q2. (n=3) (Student’s T test) (D) Lysis of target and non-target cells by TCR+ and TCR− pASP85-CAR-T cells (n=3). (ANOVA multiple-comparison). NS; not significant. All data with error bars are presented as mean ± SD. All data are representative of three or more experiments. See also Figure S3 and Table S1.