|
Antibodies
|
| Anti-Myc tag (Alexa Fluor® 647 Conjugate) |
Cell Signaling Technology |
Cat# 2233S |
| Goat polyclonal anti-human IgG |
Sigma-Aldrich |
Cat# I1886-2ML |
| CD4 Pacific Blue |
BioLegend |
Cat# 300521 |
| CD45RA APCCy7 |
BioLegend |
Cat# 304128 |
| CD45RO BV570 |
BioLegend |
Cat# 304226 |
| CD62L BV605 |
BD Biosciences |
Cat# 562719 |
| CD95 PE |
BioLegend |
Cat# 305608 |
| CD8 BV786 |
BD Biosciences |
Cat# 563823 |
| anti-Myc-Tag (9B11) Mouse mAb HRP Conjugate |
Cell Signaling Technology |
Cat# 2040S |
| anti-CD95 Clone DX2 164Dy |
Fluidigm |
Cat# 3164008B |
| anti-CTLA-4 Clone 14D3 170Er |
Fluidigm |
Cat# 3170005B |
| anti-CD62L Clone DREG56 153Eu |
Fluidigm |
Cat# 3153004B |
| anti-PD-1 Clone EH12.2H7 155Gd |
Fluidigm |
Cat# 3155009B |
| anti-CD45RO Clone UCHL1 165Ho |
Fluidigm |
Cat# 3165011B |
| anti-CD244 polyclonal 142Nd |
Novus Biologicals |
Cat# AF1039 |
| anti-CD4 Clone RPAT4 145Nd |
Fluidigm |
Cat# 3145001B |
| anti-CD8 Clone RPAT8 146Nd |
Fluidigm |
Cat# 3146001B |
| anti-EOMES clone 644730 148Nd |
Novus Biologicals |
Cat# MAB6166 |
| anti-Lag-3 Clone 11C3C65 150Nd |
Fluidigm |
Cat# 3150030B |
| anti-CD3 Clone UCHT1 170Er |
Fluidigm |
Cat# 3170001B |
| anti-TIGIT Clone MBSA43 154Sm |
Fluidigm |
Cat# 3154016B |
| anti-CCR7 Clone G043H7 159Tb |
Fluidigm |
Cat# 3159003A |
| anti-CD45 Clone HI30 89Y |
Fluidigm |
Cat# 3089003B |
| anti-Granzyme B Clone GB11 171Yb |
Fluidigm |
Cat# 3171002B |
| anti-CD57 Clone HCD57 172Yb |
Fluidigm |
Cat# 3172009B |
| T-cell receptor mimic (TCRm) monoclonal antibody |
Kim, S. et al.58
|
NA |
|
Biological Samples
|
| Primary T cells |
University of Pennsylvania Human Immunology Core. |
NA |
| CD34+ Cells |
Lonza |
Product number “Dn39444” for the experiment in Figure 5F–K, “CB21/0017/35” for the experiment in Figure S6H,I
|
|
Chemicals, peptides, and recombinant proteins
|
| anti-CD3/CD28 dynabeads |
Invitrogen |
Cat# 402.03D |
| anti-biotin microbeads |
Miltenyi |
Cat# 130-105-637 |
| HER2 beads |
Acro Biosystems |
Cat# K006 |
| PMA+ionomycin |
eBioscience |
Cat# 00-4970-93 |
| Live/Dead Aqua |
Thermo-Fischer Scientific |
Cat# L-34966 |
| RPMI 1640 |
GIBCO |
Cat# 72400-047 |
| Foetal bovine serum (FBS) |
VWR |
Cat# S170G |
| Peptides |
This manuscript |
Please see Table S2
|
| Custom p/HLA multimers |
Proimmune, Sarasota, FL |
NA |
|
Critical commercial assays
|
| Amaxa P3 Primary Cell kit and protocol |
Lonza |
Cat# V4XP-3012 |
|
Experimental Models: Cell Lines
|
| HEK 293T |
ATCC |
CRL-3216 |
| Jurkat E6.1 |
ATCC |
TIB-152 |
| CD19+ NALM6 human acute lymphoblastic leukemia |
ATCC |
CRL-3273 |
| Engineered CBG+GFP+ NALM6 human acute lymphoblastic leukemia |
University of Pennsylvania, Dr. Saar Gill |
NA |
| Parental human HER2+ ovarian cancer cell line SKOV3 |
ATCC |
HBT-77 |
| Engineered CBG+GFP+ human HER2+ ovarian cancer cell line SKOV3 |
University of Pennsylvania, |
NA |
|
Dr. Saar Gill |
|
| Parental HER2− breast cancer cell line MDA-MB-468 |
ATCC |
HBT-132 |
| Engineered CBG+GFP+ HER2− breast cancer cell line MDA-MB-468 |
University of Pennsylvania, Dr. Saar Gill |
NA |
| CD20+ Raji Burkitťs lymphoma |
ATCC |
CCL-86 |
| Engineered CBG+GFP+ Raji Burkitťs lymphoma |
University of Pennsylvania, Dr. Saar Gill |
NA |
| K562 erythro-megakaryoblastic leukemia cell line |
ATCC |
CRL-3343 |
| Engineered CD20+ K562 erythro-megakaryoblastic leukemia cell line |
University of Pennsylvania, Dr. Avery D. Posey, Jr.; Panjwani, M.K. et al.54
|
NA |
| XL1-Blue Supercompetent Cells |
Agilent Technologies |
Cat# 200236 |
|
Experimental Models: Organisms/Strains
|
| NOD/scid/IL2rg |
University of Pennsylvania Stem Cell and Xenograft Core |
RRID:IMSR_JAX:005557 |
| SGM3 (NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ |
The Jackson Laboratory |
RRID:IMSR_JAX:013062 |
|
Recombinant DNA
|
| Lentiviral construct pASP4.2 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP4.2.1 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP5 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP7 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP8 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP9 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP18 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP26 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP28 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP30 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP36 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP38 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP52 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP59 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP72 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP73 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP75 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP85 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP89 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP90 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP96 |
This manuscript |
Please see Table S1
|
| Lentiviral construct pASP97 |
This manuscript |
Please see Table S1
|
| Lentiviral construct ceGFP |
This manuscript |
Please see Table S1
|
| Lentiviral construct chIL-12 |
This manuscript |
Please see Table S1
|
|
Software and algorithms
|
| GraphPad Prism |
GraphPad Software |
https://www.graphpad.com/
|
| FlowJo |
BD |
https://www.flowjo.com/
|
| Cyt tool (R2016b) |
Columbia University |
https://www.c2b2.columbia.edu/danapeerlab/html/cyt-download.html
|
