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. Author manuscript; available in PMC: 2023 Jul 25.
Published in final edited form as: Sci Signal. 2023 May 30;16(787):eadf6696. doi: 10.1126/scisignal.adf6696

Fig. 1. Jedi T cells deplete HSCs.

Fig. 1.

(A) Representative immunohistochemical staining for GFP of Pdgfrb-GFP adult mouse liver. PV, portal vein; CV, central vein. Scale bar, 50 μm. n = 10 mice. (B) Immunofluorescence microscopy of liver section from Pdgfrb-GFP transgenic mice for GFP and desmin. Scale bar, 20 μm. n = 10 mice. (C) Experimental scheme. (D) Macroscopic liver images of control and HSC-depleted mice. Scale bar, 5 mm. (E) Liver-to-body weight ratios of control and HSC-depleted mice. Mean+SD. n=8 mice per group. (F and G) Immunofluorescence microscopy of liver sections from control and HSC-depleted mice for desmin (F) and GFP (G). Nuclei are stained with DAPI. Squares indicate areas of enlargement. Scale bars, 100 μm (lower magnification) and 20 μm (enlargements in F) and 50 μm (enlargements in G). n = 10 mice per group. (H) Quantification of desmin+ tissue area in control and HSC-depleted mice. Mean+SD. n=5 mice per group. (I and J) qRT-PCR for Des (I) and Pdgfrb (J) expression in whole-liver RNA. Mean+SD. n=5 mice per group. (K) H&E staining of sections of control and HSC-depleted livers. Scale bar, 50 μm. Arrows indicate examples of sinusoids. (L) Serum ALT in control and HSC-depleted mice. Mean+SD. n=10 control and 7 HSC-depleted mice. *p< 0.05, **p< 0.01, by unpaired two-tailed Mann-Whitney t-test.