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. 2023 Jun 9;12(7):2061–2072. doi: 10.1021/acssynbio.3c00104

Figure 2.

Figure 2

OpenCIDAR design and function. (A) Design of the new destination vectors containing KanR, the pBBR1 origin and rep gene, and a CIDAR cloning site where transcription units may be assembled. Although this is over 100 parts, only the parts in this study are shown. (B) Molecules of Equivalent Fluorescein (MEFL) expression values for the combinations of parts included in the original CIDAR paper. These were interpolated from CIDAR Figure S1. Expression from the original vector series in E. coli matches closely to the expression reported in this study. (C) Violin plots of the expression values in MEFL for E. coli populations expressing each construct, along with the average copy number assessed by ddPCR. The violin plots for three replicates are overlaid, showing little variation between replicates. (D) The same plots in as in C for the same constructs expressed in P. putida. (E) The same plots in as in C for the same constructs expressed in C. necator. (F) The same plots in as in C for the same constructs expressed in K. nataicola.