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. 2023 Jun 14;12(7):1924–1934. doi: 10.1021/acssynbio.3c00080

Figure 1.

Figure 1

Modular exchange of the EcR LBD for the GR LBD reprograms the VgEcR scaffold to respond to GR agonists. (a) Illustration of the original VgEcR/RXR system and the LBD exchange approach applied to it here. (b) The engineered VgGR/RXR pair activates luciferase reporter expression in response to the GR agonists dexamethasone (DEX) and prednisolone (PRED) in co-transfected HEK293 cells (n ≥ 5). Curve fits represent a three-parameter dose–response curve (Methods). (c) The engineered VgGR/RXR system is insensitive to PonA, in contrast to the original VgEcR/RXR system (n ≥ 8). (d) The original VgEcR/RXR pair is insensitive to DEX and PRED (n = 6). Error bars represent standard deviation. Cartoons were created with BioRender.com.