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. 2023 Jun 14;12(7):1924–1934. doi: 10.1021/acssynbio.3c00080

Figure 2.

Figure 2

Multi-input control by cooperation of GR and RXR agonists. (a) Illustration of the 2-input scheme for regulating the VgGR/RXR pair (created with BioRender.com). (b) A simultaneously present RXR agonist can boost DEX-activated responses, as observed by a two-dimensional titration of DEX vs BEX (n ≥ 5). The stark drop-off in reporter expression at the highest BEX concentration is likely due to toxicity, as a similar drop-off was also observed for constitutively expressed Renilla luciferase (Figure S7). (c) BEX shifts DEX dose–response curves to higher output levels (n = 6). (d) DEX shifts BEX dose–response curves to higher output levels (n = 6). Curve fits represent a three-parameter dose–response curve (Methods). The data points at 10 μM and 51 μM BEX were omitted for these curve fits due to the drop-off in reporter expression (see also Figure S7). Error bars represent standard deviation.