Figure 3.

Multi-input control by simultaneous presence of GR agonists and antagonists. (a) Illustration of the 3-input scheme for regulating the VgGR/RXR pair (created with BioRender.com). (b) A simultaneously present GR inhibitor can attenuate DEX-activated responses, as observed by a two-dimensional titration of DEX vs RU-486 without BEX (left) or with BEX (right) at a saturating concentration of 1 μM (Figure 2) (n ≥ 5). (c) BEX shifts RU-486 dose–response curves to higher output levels (n = 6). (d) RU-486 modulates agonist sensitivity, as observed by shifting DEX dose–response to higher concentrations (n = 6). The data point at 51 μM RU-486 is omitted from the curve fits and is likely due to toxicity, as a similar drop-off was also observed for constitutively expressed Renilla luciferase (Figure S11). Curve fits represent a three-parameter dose–response curve (Methods). Error bars represent standard deviation.