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CAST system schematic. (A) Schematic of the experimental steps for CAST-mediated transposon mutagenesis. The bacteria are first transformed with pCAST, then CAST is induced, and the strain is transformed with the suicide plasmid pDonor, which encodes for the sgRNA and the transposon. (B) The transposon inserts directionally with the left end (LE) integrating nearest the PAM.
