Figure 6.

Markers for the endolysosomal pathway and early stages of xenophagy for K1F-GFP-EGF in T24 bladder epithelial cells. (A) K1F-GFP alone stained with anti-EEA1 (B), Anti-Rab7 (C), Anti-Cathepsin-L (D), or Anti-Galectin-8. In all cases, 1 × 10⁸ K1F-GFP were incubated with T24 cells for 1 h and stained with the respective antibodies. DAPI is shown in blue, and antibodies for the respective markers are shown in gray. (E) Quantification of colocalization with respective cellular markers for K1F-GFP and K1F-GFP-EGF alone. A total of 10 FOV images were quantified for each phage and for each marker in n = 3 experiments. At least 100 cells were enumerated per replicate for each marker. A Student’s t-test corrected for multiple comparisons was used to compute significance between phage types for each marker.