Figure 7.

Sucrose inhibition of K1F variants. T24 cells were exposed to 0.5 M hypertonic sucrose for 10 min and 1 × 10⁸ PFU of K1F-GFP or K1F-GFP-EGF were added to the cells and incubated for 1 h before fixation. (A) Immunofluorescence of uninhibited K1F-GFP; (B) K1F-GFP treated with sucrose; (C) uninhibited K1F-GFP-EGF; (D) K1F-GFP-EGF treated with sucrose; (E) quantification of inhibition assays. Quantification was performed via thirty FOV images, where at least 250 cells were enumerated per condition. A two-way ANOVA with post-hoc Tukey tests was performed to test for significance between treatments.