Fig. 6.

USP16 is elevated and contributes to gouty arthritis in vitro. A The mRNA level of USP16 was detected by RT-qPCR. B The protein levels of USP16 and Drp1 in RAW264.7 and J774A.1 cells were detected by western blot. C The secretion of IL-1β, TNF-α and IL-6 were measured by ELISA assay. D The activities of antioxidant enzymes were detected using commercial kits. E The LDH release was measured using commercial kit. F Mitochondrial ROS was assessed by MitoROX staining. Red, MitoROX. Scale bar = 100 μm. *, P < 0.05, **, P < 0.01, ***, P < 0.001