Fig. 7.

USP16 induces gouty arthritis via Drp1-dependent NLRP3 inflammasome activation. A The protein levels of USP16 and Drp1 was detected by western blot. B The protein levels of the key molecules of NF-κB/NLRP3 signaling were detected by western blot. C The secretion of IL-1β, TNF-α and IL-6 were measured by ELISA assay. D The activities of antioxidant enzymes were detected using commercial kits. E The LDH release was measured using commercial kit. F Mitochondrial ROS was assessed by MitoROX staining. Red, MitoROX. Scale bar = 100 μm. *, P < 0.05, **, P < 0.01, ***, P < 0.001