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. 2023 Jul 24;52:47. doi: 10.1186/s40463-023-00647-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — A Endoscopic image at injection. Saline or different doses of basic fibroblast growth factor (bFGF) (200 and 2000 ng: low and high dose, respectively) were injected into the left thyroarytenoid (TA) muscle using laryngeal endoscopy under general anesthesia. B Experimental timeline. The left recurrent laryngeal nerve (RLN) of rats was transected on day-1. Saline or different doses of bFGF were injected into the left TA muscle transorally on day 0. The laryngeal tissues were collected on the indicated days. C, D Representative images of hematoxylin and eosin-stained sections of the larynx 1 day after left RLN transection (C, 40 × magnification; D, 400 × magnification). The solid and dotted rectangles in (C) indicate the TA region of the muscle of the untreated and treated sides, which are shown at a higher magnification in (D)