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. 2023 Jul 24;14:180. doi: 10.1186/s13287-023-03408-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Identification of MSC-sEV. MSC-sEVs were isolated from iPSC-MSCs using anion-exchange chromatography. A. Nanoparticle Tracking Analysis (NTA) showed the particle size distribution and concentration of MSC-sEVs. B. TEM showed morphology and size of MSC-sEVs. C. CD9, CD63 and calnexin expression in MSCs and sEVs were determined by western blotting. The original blots are presented in Additional file 1: Fig. S4. MSC: mesenchymal stromal cells; MW: molecular weight; sEV: small extracellular vesicles; TEM: transmission electron microscope. Scale bar, 200 nm