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. 2023 Jul 24;24(1):93. doi: 10.1186/s10194-023-01627-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Experimental Design. Mechanical thresholds of CTRL (5% or 10% ethanol in saline) and LVC (1 mg/kg) groups were tested at baseline (BL), 30^th, 60^th, 90^th, 120^th, 180^th and 240^th min time points. NTG (10 mg/kg) group was tested at baseline, 60^th, 120^th, 180^th and 240.^th min. Timeframes with lowest thresholds were accepted as “headache phase” and all other experimental procedures were coincided to that timeframe (30-90 min for LVC and 120-180 min for NTG)