Fig. 2.
Screening of scFv against the U1 epitope. A In the fourth round of biopanning, approximately 400 individual scFv phages were selected, and the monoclonal phage ELISA was performed. Based on absorbance cutoff (O.D.490 nm= 2.0), 72 high binder scFv phages were selected, and each phage was quantified. B After quantification of scFv phages, we picked phages in equal numbers (1 × 109) to perform the monoclonal phage ELISA. The top 24 scFv phages were selected based on absorbance cutoff (O.D.490 nm = 2.4). C Sequencing of all scFvs revealed only 4 unique clones with sequence diversity in CDRs. Residues highlighted in red color are the same in all scFvs. All experiments were done in triplicate. The error bars represent standard deviations