Fig. 6.

Virus-like particles are produced by Pg cultures. Transmission electron micrograph (2.73 pixels/nm) of the ATCC 49417 cell-free, ultracentrifuged supernatant that, when sequenced, produced the reads mapped in Fig. 5; virus-like particles were sparse among likely extracellular vesicles, despite high coverage of the prophage region in DNA from this material (A). Magnification (5.52 pixels/nm) of the virus-like particle from panel A (B). Transmission electron micrograph (2.25 pixels/nm) of supernatants of a 3-day-aged ATCC 49417 culture derived from passages of the same stock that gave rise to the cultures imaged in panels A and B; virus-like particles were more abundant than in ultracentrifuge pellets shown in panels A and B and more commonly showed angular, icosahedral capsids (C). Magnification (8.81 pixels/nm) of the virus-like particle from panel C (D)