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Effect of formaldehyde on the growth of B. subtilis. Overnight cultures of wild-type strain 168 (▄), YD101 (yckG) (•••••), and YD102 (yckF) (–––) in LB medium were inoculated into prewarmed LB medium containing formaldehyde at concentrations of 0.0 (a), 0.3 (b), 0.6 (c), and 0.9 (d) mM. Growth and lysis of cells at 37°C were monitored automatically at an OD of 660 nm.
