TABLE 1.

HPS and PHI activity in E. coli bearing an expression plasmid

Straina	Sp act (nmol of NADP reduced/min/mg of protein)b in cell extracts
	HPS	PHI	HPS + PHIc
E. coli JM109/pT-Bsb-yckG6	3,160	<1	NDd
E. coli JM109/pT-Bsb-yckF1	<1	25,900	ND
E. coli JM109/pT-Bsb-yckGF1	ND	ND	3,970
E. coli JM109/pT-Bs-ΔNcoe	<1	<1	ND

^aCells were grown in modified M9 Casamino Acids medium with indoleacrylic acid. 

^bMean value from three experiments. 

^cTotal activity for serial reaction of HPS and PHI. 

^dND, not determined. 

^eNegative control.