TABLE 2.
HPS and PHI activities in B. subtilis 168 and deletion mutants under different growth conditions
| Strain | Growth conditionsa | Sp act (nmol of NADPH produced/ min/mg of protein)b in cell extracts
|
||
|---|---|---|---|---|
| HPS | PHI | HPS + PHI | ||
| 168 wild type | LB | <1 | <1 | NDc |
| LB + 0.5 mM FA | 690 | ND | 310 | |
| LB + 1.0 mM FA | 95 | ND | <1 | |
| LB + 2.0 mM FA | <1 | ND | <1 | |
| LB + 2% (vol/vol) MeOH | <1 | ND | <1 | |
| LB + 0.5 mM FM | <1 | ND | <1 | |
| YD101 (yckG) | LB + 0.5 mM FA | <1 | <1 | ND |
| YD102 (yckF) | LB + 0.5 mM FA | 290 | <1 | ND |
| YD111 (yckH) | LB + 0.5 mM FA | <1 | <1 | <1 |
| YD112 (yckH) | LB + 0.5 mM FA | <1 | <1 | <1 |
| YD121 (yckH yckH+) | LB + 0.5 mM FA | 1,050 | ND | 200 |
a
FA, formaldehyde; MeOH, methanol; FM, formate.
b
Mean value from three experiments.
c
ND, not determined.