Fig. 6.

Signal inhibitors reversed the effects of leptin on LPS/IFN-γ-induced M1 macrophages. a Western blot analysis of iNOS and phosphorylation levels of obR-b and members of the downstream JNK/STAT3/AKT pathways in BMDMs. b The displayed protein expression value is the ratio of phosphorylated quantitative protein or iNOS protein to total protein. c Quantitative analysis of CD86 in BMDMs treated with or without Allo-Aca. d Quantitative analysis of CD86 in BMDMs treated with or without different synthetic inhibitors. e Representative histogram of CD86 expression (left) and quantitative analysis of CD86 (right) expression in female db/db mouse-derived BMDMs. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, each experiment was performed three times