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. 2023 Jul 24;29:100. doi: 10.1186/s10020-023-00702-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — The effects of leptin/obR signaling on neutrophil chemotaxis. a Real-time PCR of CXCL2 mRNA levels normalized to those of GAPDH. b CXCL2 protein levels were analyzed by ELISA. c Real-time PCR of CXCL2 mRNA levels normalized to those of GAPDH. d CXCL2 protein levels were analyzed by ELISA. e Transwell assay to detect neutrophil chemotaxis (×100). f Chemotactic index of neutrophils. g Transwell assay to detect neutrophil chemotaxis (×100). h Chemotactic index of neutrophils. Each experiment was performed three times. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001