Table 3.

ANOVA table for response surface quadratic model for biomass yield and protein concentration

Variable	Response
	Protein (%)						Biomass dry weight (g/L)
Source	Sum of squares	df	Mean square	F value	p-value		Sum of squares	df	Mean square	F value	p-value
Model	765.07	14	54.65	2.50	0.0406a	Significant	0.1675	10	0.1675	3.57	0.0074a	Significant
A-NaNO3	43.31	1	43.31	1.98	0.1780		0.0004	1	0.0004	0.088	0.768
B-NaHCO3	131.70	1	131.70	6.03	0.0258a	Significant	0.0113	1	0.0113	2.40	0.136
C-BFCE	34.46	1	34.46	1.58	0.226		0.04	1	0.04	9.98	0.004a	Significant
D-pH	43.74	1	43.74	2.00	0.176		0.0258	1	0.0258	5.40	0.030a	Significant
A2	0.0312	1	0.0312	0.0014	0.970		0.000733	1	0.000733	0.16	0.69
B2	144.70	1	144.70	6.63	0.0203a	Significant	0.0053	1	0.0053	1.2	0.289
C2	3.12	1	3.12	0.143	0.710		0.0036	1	0.0036	0.81	0.382
D2	64.73	1	64.73	2.97	0.104		0.0149	1	0.0149	3.36	0.085
A * B	101.00	1	101.00	4.63	0.047a	Significant	0.0012	1	0.0012	0.26	0.614
A * C	98.51	1	98.51	4.51	0.049a	Significant	0.0441	1	0.0441	9.40	0.0061
A * D	46.72	1	46.72	2.14	0.162		0.0006	1	0.0006	0.13	0.718
B * C	24.21	1	24.21	1.11	0.307		0.0121	1	0.0121	2.58	0.123
B * D	26.42	1	26.42	1.21	0.287		0.0000	1	0.0000	0.005	0.942
C * D	6.79	1	6.79	0.311	0.584		0.0256	1	0.0256	5.46	0.030a	Significant
Residual	349.15	16	21.82					20
Lack of fit	277.05	10	27.70	2.31	0.1594	Not significant		14		1.76	0.2500	Not significant
Pure Error	72.11	6	12.02					6
Core total	1114.22	30						30
R2 = 70.59%			Adjusted R2 = 44.85%				R2 = 72.58%		Adjusted R2 = 48.58%

^aSignificant value