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. 2023 Jul 13;2(7):pgad231. doi: 10.1093/pnasnexus/pgad231

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© The Author(s) 2023. Published by Oxford University Press on behalf of National Academy of Sciences.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Tubulin colocalization and microtubule organization with MAP65 condensates. A) Tubulin co-localizes with MAP65 liquid droplets. (i) Representative confocal image of rhodamine tubulin (red, left) and GFP-MAP65 (green, center) and overlay (merge, right) in a liquid-like droplet. Scale bar is 5 $μ$ m. (ii) Intensity scan through the droplet in the rhodamine channel normalized so that the intensity outside is one. B) Tubulin co-localizes with MAP65 matured droplets. (i) Representative confocal images of rhodamine tubulin (red, left) and GFP-MAP65 (green, center) and overlay (merge, right) in a gel-like droplet. Scale bar is 5 $μ$ m. (ii) Intensity scan through the droplet in the rhoamdine channel normalized so that the intensity outside the droplet is one.