TABLE 1.
Isocitrate lyase activity in M. avium grown on various carbon substratesa
Carbon source | Mean enzyme sp act (nmol/min/mg of protein) ± SD |
---|---|
Palmitate | 1,193.4 ± 0.2 |
Acetate | 439.6 ± 0.2 |
Glucose | 97.6 ± 0.2 |
Succinate | 12.3 ± 0.35 |
Acetate-glucose | 84.7 ± 0.35 |
Acetate-succinate | 17 ± 0.5 |
Palmitate-glucose | 217.5 ± 0.51 |
Palmitate-succinate | ≤5 |
Middlebrook medium | 120.5 ± 0.5 |
Cell extracts were made from cultures grown on the indicated carbon source and assayed for enzyme activity. The values represent the mean of at least three independent experiments.