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. 2023 Jul 9;19(11):3576–3594. doi: 10.7150/ijbs.85133

Figure 1.

Figure 1

P2RY12 deficiency protected from ConA-induced immune hepatitis. (A) Schematic diagram of ConA-induced immune hepatitis mouse model. WT and P2RY12-/- mice were administered ConA (12.5 mg/kg body weight via i.v. injection) for 12 hr (left). The livers were harvested and pictured from WT after saline for 12 hr, WT and P2RY12-/- mice after 12.5 mg/kg ConA injection for 12 hr (right). (B) Serum levels of AST and ALT in WT mice and P2RY12-/- mice were detected. (C) Photomicrographs of representative H&E-stained mouse livers. Massive hepatocyte necrosis (dark arrows) was observed in WT+ConA group. (D) ELISA analysis of cytokines (IFN-γ, IL-6, IL-12p70, TNF-α and IL-10) in the serum from WT and P2RY12-/- mice without or with 12.5 mg/kg ConA injection for 12 hr. (E) mRNA levels of Ifng, Il6, Il12a, Tnf and Il10 in liver tissue from WT and P2RY12-/- mice were assessed. (F) Liver cleaved caspase-3, JNK and p-JNK protein levels were determined by Western blot (left). Pooled data are presented in the right panel. Data represented as the mean±SD of 4 mice per group. Data are mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 vs indicated group. (two-tailed Student's t-test).