Figure 7.

P2RY12 medicated the expression of IFN-γ and HK2 stability in AIH patients and healthy human PBMCs stimulated by ConA in vitro. (A) Healthy human lymphocytes were obtained and cultured for 24 hr in an environment containing 2 μg/ml ConA and treated with different doses of clopidogrel and ticagrelor (1, 3, 10 μM). Flow cytometric analysis of IFN-γ⁺ in gated CD4⁺ T cells and CD8⁺ T cells. (B, C) Flow cytometric analysis of HK2 in CD4⁺ T cells and CD8⁺ T cells stimulated for 24 hr with ConA followed by incubation with different doses of clopidogrel and ticagrelor. (D) Flow cytometric analysis of IFN-γ⁺ cells in gated CD4⁺ T cells and CD8⁺ T cells in ConA-stimulated human PBMCs treated with 3-BrPA. (E) Flow cytometric analysis of intracellular HK2 in human PBMCs stimulated for 24 hr with ConA followed by incubation with P2RY12 inhibitors (3 μM Clopidogrel or Ticagrelor) or with SC79 for 6 hr. (F) Flow cytometric analysis of IFN-γ⁺ cells in gated CD4⁺ T cells or CD8⁺ T cells in AIH patients PBMCs followed by incubation with P2RY12 inhibitors (3 μM Clopidogrel or Ticagrelor) for 24 hr. (G) Pooled data presented from (F). *P< 0.05; **P< 0.01; ***P < 0.001 vs indicated group. Data are representative of three independent experiments. Summary data are shown as mean ± SEM. with P values determined by two-tailed Student's t test.