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. 2023 Jul 15;19(11):3628–3639. doi: 10.7150/ijbs.82933

Figure 3.

Figure 3

11β-HSD1 promotes osteoclastogenesis in vitro. A. BMMs were isolated from the wild type, KO and KI groups. After induction with osteoclastic differentiation medium for 7 days, TRAP staining was performed to evaluate osteoclast formation. B. Western blot (WB) evaluation of 11β-HSD1 and CTSK expression change in BMMs from the wild type, KO and KI groups. C. RT-qPCR analysis of 11β-HSD1, ACP5, Cath-K and Nfatc-1. D. BVT-2733 with different concentrations were added into osteoclast incubation medium. Trap staining was performed to evaluate the osteoclast formation. (*means P value<0.05, **means P value<0.01)