Figure 2.

Distinct types of liver organoids. Monocellular liver organoids such as HOs and COs can be generated from either liver tissue or hPSCs. Multi-tissue liver organoids containing both parenchymal and non-parenchymal cell types can be generated by multiple ways such as by unguided differentiation, guided differentiation with multi-lineage differentiation cues, and coculture of parenchymal- and non-parenchymal-supporting cell types. Multi-organ liver organoids with proper inter-organ crosstalk can also be generated by either assembloid or organ-on-chip technology.