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. 2023 Jun 26;19(11):3324–3340. doi: 10.7150/ijbs.80979

Figure 2.

Figure 2

The effects of SMURF2 on the expression of cell cycle proteins in lung cancer cell lines. (A) Biological processes enrichment analysis of the target genes co-expressed with SMURF2. (B-D) RT-qPCR and WB analysis of SMURF2 expression in SMURF2-KD A549 (b) and H460 (c) cells. (E, F) Detection of mRNA level of p18, p21, p27, CDK2, CDK4 and CDK6 in SMURF2-KD A549 (e) and H460 (f) cells. (G, H) SMURF2-OE efficiency of A549 cell was detected by RT-qPCR (g) and WB (h). (I) Detection of mRNA level of p21, p27, CDK2 and CDK4 in SMURF2-OE A549 cells. (J, K) SMURF2-OE efficiency of H460 cell was detected by RT-qPCR (j) and WB (k). (L) Detection of mRNA level of p21, p27, CDK2 and CDK4 in SMURF2-OE H460 cells. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, no significance.