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. 2023 Jun 26;19(11):3324–3340. doi: 10.7150/ijbs.80979

Figure 3.

Figure 3

SMURF2 knockdown promotes cell proliferation and tumorigenesis. (A, B) Cell proliferation was monitored by CCK-8 assay of A549 and H460 cells at the indicated time points (0 h, 24 h, 48 h and 72 h). (C) A549 cell cycle was determined by flow cytometry with quantitative analysis (right panel). (D) H460 cell cycle was determined by flow cytometry with quantitative analysis (right panel). (E) Cell migration of A549 cells were assessed by wound healing assay with quantitative analysis (bottom panel). (F) Cell migration of H460 cells were assessed by wound healing assay with quantitative analysis (bottom panel). (G) Migration and invasion of A549 cells were monitored by transwell assay with quantitative analysis (right panel). (H) Migration and invasion of H460 cells were monitored by transwell assay with quantitative analysis (right panel). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, no significance; scale bar, 100 μm.