Figure 10.

miR-221 regulate MAPK pathway and PI3K/AKT pathway via targeting IGF-1. (A) Western blot experiments were used to analyze the expression of relevant proteins in MAPK pathway and PI3K/AKT pathway in DPCs and DSCs respectively after treatments of miR-221, miR-221 + IGF-1 and miR-NC + IGF-1. CCK-8 assay (B), EdU assay (C) and cell cycle assay (D) were performed after DPCs treated with IGF-1, IGF-1 + PD98059 and vehicle, as well as DSCs treated with IGF-1, IGF-1 + LY294002 and vehicle, Student's t-test, mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001. Scale bars represent 100 μm in (C).