Skip to main content
. 2023 Jul 9;19(11):3544–3557. doi: 10.7150/ijbs.82566

Figure 6.

Figure 6

MDM2 inhibition induces mitochondria-mediated apoptosis in PTEN-deficient CRC cells. HCT116 (A) and RKO (C) PTEN-isogenic cells were treated with MDM2 inhibitor (CGM097) for 72 h, then labelled with Annexin V-Alexa Flour 488 (AF488) and propidium iodide. The flow cytometry data (A, C) and apoptotic cells quantitative data (B, D) are shown. All data were represented as mean± SD, *P < 0.01. E, F. Western blot analysis represented cleaved caspase 3 in HCT116 (E) and RKO (F) PTEN-isogenic cells in respond to CGM097 for 48 h. G. The mitochondrial membrane potential of HCT116 PTEN-isogenic cells were measured by JC1 staining after CGM097 treatment. CCCP served as a positive control for reduction in mitochondrial membrane potential. H. Effect of CGM097 on cellular distribution of cytochrome c (green) in HCT116 PTEN-/- cells. The nucleuses were labelled with DAPI (blue).