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. 2023 Jul 3;19(11):3428–3440. doi: 10.7150/ijbs.84426

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Lats2 silencing prevents ROS generation, mitochondrial membrane depolarization, and mitochondria-dependent apoptosis in hypoxic cardiomyocytes. Measurements were made in cultured HL-1 cardiomyocytes 48 h after hypoxia treatment. (A, B) DCFDA fluorescence assay was used to determine the production of cellular ROS. (C, D) Analysis of changes in mitochondrial membrane potential (ratio of red-to-green fluorescence intensity) in cells loaded with JC-1. (E, F) Immunofluorescence staining of cyt-c. (G) Measurement of mPTP opening rate. (H, I) Analysis of caspase-3 activity and caspase-9 concentration. #p<0.05.