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. 2023 Jul 3;19(11):3428–3440. doi: 10.7150/ijbs.84426

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Ablation of Lats2 attenuates myocardial infarction-mediated mitochondrial fission. (A-C) RT-qPCR analysis of the transcription of Drp1, Mff, and Fis1 in mouse heart. (D-F) Immunofluorescent detection of mitochondria and quantification of mitochondria average length and ratio of HL-1 cells with fragmented vs tubular mitochondria. (G) ELISA-based analysis of ATP production in HL-1 cells pre-treated with FCCP. (H, I) Analysis of mitochondrial respiration function in HL-1 cells pre-treated with FCCP. (J, K) Analysis of ROS generation in FCCP pre-treated, DCFDA-loaded HL-1 cells. (L) Quantification of mPTP opening time in HL-1 cells pre-treated with FCCP. (M, N) Analysis of caspase 3 activity and caspase 9 concentration in FCCP-treated HL-1 cells. #p<0.05.