FIG 11.

KB2B1 readily forms a biofilm on all LBS media but not all tTBS media. (A) Colony biofilm formation was evaluated following growth on LBS (L), LBS+calcium (LC), LBS+pABA (LP), LBS+pABA/calcium (LPC), tTBS (T), TC, TP, and TPC of WT KB2B1. Pictures were taken using the Zeiss Stemi 2000-c microscope (×6.5 magnification) at 72 h before and after disruption using a toothpick. Pictures are representative of three separate experiments. Arrows indicate where “pulling,” indicating cohesion, was observed. (B) Biofilm cohesion was quantified from images as described in Materials and Methods with scores of 1 assigned to a null phenotype and 4 to the most/strongest. Statistics were performed via a one-way ANOVA using Tukey’s multiple-comparison test, where biofilm strength was the dependent variable. **, P = 0.0025.