FIG 2.
OprF affects late-stage biofilms in TSB. (A) 1-h static microtiter biofilm assays were performed in TSB with PAO1 (WT, black), ΔpslD (blue), ΔoprF (red), and a ΔoprF attTn7::PBAD-oprF restoration strain (ΔoprF + oprF) with (white) and without (gray) 0.5% arabinose (ara). (B to E) 4-h (B), 8-h (C), 16-h (D), and 24-h (E) assays were performed with the same strains and media. (F) Static ΔoprF biofilm formation relative to WT (dashed line) at respective time points is represented. Biofilm formation is normalized to WT in each respective medium. Error bars, SEM (n = 3); dot, each biological replicate, which is the average of 3 to 6 technical replicates; letters, statistical groupings (P < 0.01, one-way ANOVA with post hoc Tukey HSD); asterisk, statistically different from WT at the same time point.