FIG 4.
ΔoprF exhibits no biofilm cell viability defect in TSB. Side-by-side 24-h static microtiter biofilms (A to C, solid bars) and biofilm cell viability assays (D to F, outlined bars) were performed in the same 96-well plate with P. aeruginosa PAO1 (WT, black), ΔoprF (red), and the ΔoprF attTn7::PBAD-oprF restoration strain with 0.5% arabinose (ΔoprF + oprF + ara; gray). Biofilms were grown in TSB (A and D), LB (B and E), or TSB with 10 g/L NaCl (C and F). Static biofilm formation (A to C) is normalized to WT. Error bars, SEM (n = 3); dot, each biological replicate, which is the average of 5 technical replicates; asterisks, statistical difference from PAO1 (P < 0.05, one-way ANOVA with post hoc Tukey HSD).