TABLE 1.
Mutant alleles characterized in this study
| pilT allele | Phenotypea | Reference(s) |
|---|---|---|
| D31K | Decreased stability, increased twitching, decreased closed conformation, partial phage sensitivity | 32 |
| K58A | Increase in open conformation, increased twitching, increased OOCOOC hexamer conformation | 32 |
| R123D | Decreased stability of OOOOOO and CCCCCC hexameric conformations | 32 |
| K136A (WA) | Cannot bind ATP | 29, 36 |
| E204A (WB) | Cannot hydrolyze ATP | 36 |
| T216R | Eliminates OOOOOO hexameric conformation | 32 |
| H222A | Decreased twitching, phage sensitive, decreased retraction velocity, increase in falling off T4P complex | 33, 41 |
| H229A | No twitching, phage sensitive, decreased ATPase in vitro | 32, 33 |
a
OOCOOC indicates open, open, closed, open, open, closed for the conformation of the 6 hexamers of PilT. OOOOOO or CCCCCC indicates all open or all closed conformations, respectively, of the 6 hexamers of PilT.