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. 2023 Aug 1;32(8):e4707. doi: 10.1002/pro.4707

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© 2023 The Authors. Protein Science published by Wiley Periodicals LLC on behalf of The Protein Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Constructs respond to denaturants to different extents. Concentrations of urea were 0, 2, 4, 6 M, colored from darkest to lightest line colors. Plots show 0, 2, 4, 6 M GdnHCl using the same color scheme. Aap‐PGR data were replotted from a previous publication for ease of comparison (Yarawsky et al., 2017).